1
Column selection
Start with analyte polarity, ionization, molecular size, matrix and desired selectivity. Screen complementary families rather than only changing dimensions.
A structured guide to column and condition selection.
Start with analyte polarity, ionization, molecular size, matrix and desired selectivity. Screen complementary families rather than only changing dimensions.
Choose organic modifier, aqueous component, pH and additives within stationary-phase, analyte and detector compatibility.
Scale flow approximately with column cross-sectional area while respecting pressure and efficiency.
Use controlled temperature to improve reproducibility and explore selectivity only within validated limits.
Keep injection solvent strength and volume compatible with the starting mobile phase and column dimensions.
Use gradient scouting for broad polarity ranges, then optimize slope, range and re-equilibration.
Choose isocratic operation when retention and resolution are stable across the target set.
Filter or centrifuge samples, manage matrix load and avoid solvent mismatch.
A practical customer guide to chemistry, particle size, dimensions and application requirements.
Read Technical ArticleHow USP stationary-phase categories support compendial method transfer and equivalent-column selection.
Read Technical ArticlePractical steps to reduce contamination, pressure increase and premature column failure.
Read Technical ArticleA cause-and-solution guide for improving peak symmetry.
Read Technical Article