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Manufacturer

Nomura Chemical Co., Ltd.

Develosil chromatography columns are manufactured by Nomura Chemical Co., Ltd. The phases below are shown under their applicable USP L classifications to help laboratories compare chemistries and select suitable columns for method transfer.

USP reference

USP Listing Table

USP L Packing	Develosil Column / Phase	Stationary Phase Type	USP Packing Description	Manufacturer
L1	Develosil ODS	C18 / ODS reversed phase	Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, generally 1.5–10 µm, or supplied as a monolithic rod.	Nomura Chemical
L10	Develosil CN-UG	Cyano (CN), reversed- or normal-phase use	Nitrile groups chemically bonded to porous silica particles, generally 1.5–10 µm, or supplied as a monolithic silica rod.	Nomura Chemical
L13	Develosil TMS-UG C1	C1 / trimethylsilane, low-retention reversed phase	Trimethylsilane chemically bonded to porous silica particles, generally 3–10 µm.	Nomura Chemical
L62	Develosil C30	C30, shape-selective reversed phase	C30 silane bonded phase on fully porous spherical silica, generally 3–15 µm.	Nomura Chemical
L62	Develosil C30-UG	C30, UG series	C30 silane bonded phase on fully porous spherical silica, generally 3–15 µm.	Nomura Chemical
L62	Develosil RP-Aqueous	Aqueous-compatible reversed phase	C30 silane bonded phase on fully porous spherical silica, generally 3–15 µm.	Nomura Chemical
L62	Develosil Combi-RP	Hybrid-selectivity reversed phase	C30 silane bonded phase on fully porous spherical silica, generally 3–15 µm.	Nomura Chemical
L62	Develosil RP-Aqueous-AR	Aqueous-compatible reversed phase, AR variant	C30 silane bonded phase on fully porous spherical silica, generally 3–15 µm.	Nomura Chemical

When transferring a USP method, match the USP chemistry first, then confirm particle size, internal diameter and column length against the method requirements.

Column selection guidance

What the USP Packings Mean for Develosil Selection

USP L1 – Develosil ODS

Chemistry: C18 / octadecylsilane bonded phase.

Develosil ODS is the standard reversed-phase starting point for routine assay, impurity profiling and stability testing. It provides strong hydrophobic retention and broad applicability across many compound classes.

Best suited for: General reversed-phase separations, pharmaceutical QC and stability studies
Method transfer: Match L1 chemistry before matching particle size and dimensions
Consider another phase when: More polar retention or different selectivity is required

View Develosil ODS Columns

USP L10 – Develosil CN-UG

Chemistry: Cyano / nitrile bonded phase.

Develosil CN-UG offers a useful selectivity change compared with C18. Depending on mobile-phase selection, it may be used in reversed-phase or normal-phase workflows and is useful when a medium-polarity surface is required.

Best suited for: Isomer separations, moderately polar analytes and orthogonal screening
Typical benefit: Different elution order compared with ODS
Method-development role: Alternative selectivity for aromatic and polar compounds

USP L13 – Develosil TMS-UG C1

Chemistry: C1 / trimethylsilane bonded phase.

Develosil TMS-UG C1 provides lower hydrophobic retention than C18. It is useful when strongly non-polar compounds are retained too long on conventional ODS columns or when shorter run times are required.

Best suited for: Faster analysis of non-polar compounds
Typical benefit: Reduced retention of highly hydrophobic analytes
Method-transfer role: A weaker reversed-phase alternative to C18

USP L62 – Develosil C30 Family and Related Phases

Chemistry: C30 bonded phase on fully porous spherical silica.

USP L62 phases provide enhanced shape selectivity and are particularly useful for carotenoids, lipid isomers and other geometrical or positional isomers where C18 may not offer sufficient resolution.

Develosil C30: Primary C30 option for isomeric separations
Develosil C30-UG: UG-series C30 option for reproducible performance
RP-Aqueous / RP-Aqueous-AR: Suitable for highly aqueous mobile phases and polar analytes
Combi-RP: Alternative reversed-phase selectivity for challenging matrices

View C30 Columns View RP-Aqueous Columns

Customer guidance

Frequently Asked Questions

Who manufactures Develosil columns?

Develosil chromatography columns are manufactured by Nomura Chemical Co., Ltd.

Which USP L packing applies to Develosil ODS?

Develosil ODS is classified under USP L1, the C18 / octadecylsilane bonded-phase category.

Which USP L packing applies to Develosil CN-UG?

Develosil CN-UG is classified under USP L10, the cyano / nitrile bonded-phase category.

Which USP L packing applies to Develosil TMS-UG C1?

Develosil TMS-UG C1 is classified under USP L13, the trimethylsilane / C1 bonded-phase category.

Which USP L packing applies to Develosil C30?

Develosil C30 and related C30 phases are classified under USP L62.

How should I choose an equivalent column for a USP method?

First match the USP L classification and stationary-phase chemistry. Then compare particle size, internal diameter, column length and method-specific operating conditions before final selection.

Need Help Selecting a USP-Equivalent Develosil Column?

Share the USP L classification, current column dimensions, particle size and application details with Akira Analytical Solutions Pvt. Ltd. for technical guidance.

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