1
High Backpressure
Check frit blockage, sample particulates, precipitated buffer, tubing restrictions and solvent viscosity.
Diagnose common HPLC symptoms without risking the column.
Check frit blockage, sample particulates, precipitated buffer, tubing restrictions and solvent viscosity.
Evaluate active sites, pH, ionic strength, overload, extra-column effects and column contamination.
Check injection solvent mismatch, voids, blocked frits, connection quality and sample solubility.
Confirm mobile-phase composition, pH, temperature, flow, equilibration and buffer preparation.
Assess dead volume, low efficiency, overload, slow mass transfer and unsuitable flow.
Run blanks, inspect carryover, solvent contamination, injector wash and system memory.
Use a solvent sequence compatible with the phase and contaminants; never precipitate buffer.
Remove incompatible buffers, transition to a suitable storage solvent and cap the column.
Use filtration, guard protection, controlled pH, clean solvents and pressure trending.