Manufacturer: Nomura Chemical Co., Ltd.India distributor: Akira Analytical Solutions Pvt. Ltd.

Application Note: PAH Analysis

Column and method-development guidance for polycyclic aromatic hydrocarbon separations.

This application note provides a method-development framework. Final conditions must be validated with the actual analytes, matrix, detector and laboratory system.

Recommended chemistry

Develosil PAHS is the specialist family for polycyclic aromatic hydrocarbon selectivity. C30 may be evaluated as an alternative when highly hydrophobic retention or shape selectivity is useful.

Sample cleanliness

Environmental and petroleum-derived samples can contain strongly retained matrix components. Use appropriate cleanup and filtration to reduce fouling, and consider a guard column for routine sample sequences.

Gradient development

Begin with an aqueous-organic gradient and optimize the stronger organic portion for late-eluting PAHs. Pay close attention to solvent purity and blank performance because trace contamination can produce ghost peaks.

System suitability

Monitor resolution of critical PAH pairs, retention repeatability, background response and pressure. Establish a defined wash step to remove strongly retained material between batches.

Column selection

Recommended Develosil Starting Points

Develosil C30-UG

View product information, selection guidance and available configurations.

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