Manufacturer: Nomura Chemical Co., Ltd.India distributor: Akira Analytical Solutions Pvt. Ltd.

Application Note: LC–MS Method Development

Column and mobile-phase choices for sensitive LC–MS analysis.

This application note provides a method-development framework. Final conditions must be validated with the actual analytes, matrix, detector and laboratory system.

Volatile mobile phases

Use volatile buffers and additives compatible with the selected ionization mode. Avoid non-volatile salts that can contaminate the source or suppress signal.

Column dimensions

Narrow-bore and shorter columns can reduce solvent consumption and support faster analysis, provided extra-column volume is controlled. Match particle size to the pressure capability of the LC system.

Retention and sensitivity

Adequate retention helps separate analytes from matrix components that may cause ion suppression. Evaluate aqueous-compatible phases for polar compounds and C18 phases for broad reversed-phase coverage.

Carryover control

Optimize needle wash, strong-solvent rinse and column wash steps. Verify blank response after high-concentration samples and strongly retained matrices.

Column selection

Recommended Develosil Starting Points

FireFlex C18

View product information, selection guidance and available configurations.

Fusion C18

View product information, selection guidance and available configurations.

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