Why C30
Carotenoids are highly hydrophobic and may occur as closely related geometric isomers. C30 phases provide stronger shape selectivity than conventional C18 and are therefore a strong starting point for complex carotenoid profiles.
Practical guidance for resolving carotenoids and geometric isomers using C30 selectivity.
Carotenoids are highly hydrophobic and may occur as closely related geometric isomers. C30 phases provide stronger shape selectivity than conventional C18 and are therefore a strong starting point for complex carotenoid profiles.
Use solvent systems capable of maintaining carotenoid solubility while providing controlled selectivity. Methanol, acetonitrile and stronger non-polar modifiers may be evaluated according to sample composition and instrument compatibility.
Carotenoids can be sensitive to heat, oxygen and light. Prepare samples promptly, minimize exposure and maintain a controlled column temperature to improve retention-time and selectivity consistency.
Use a shallower gradient around critical isomer pairs, reduce flow where practical and avoid excessive injection volume. Confirm system dwell volume before transferring a gradient method.
View product information, selection guidance and available configurations.
View product information, selection guidance and available configurations.
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