Why conventional C18 can struggle
Highly polar compounds may elute close to the solvent front on conventional C18, especially under water-rich conditions. An aqueous-compatible reversed-phase family can provide more stable retention.
Strategies for improving retention and peak shape for polar analytes.
Highly polar compounds may elute close to the solvent front on conventional C18, especially under water-rich conditions. An aqueous-compatible reversed-phase family can provide more stable retention.
Begin with a high proportion of water and only enough organic modifier to maintain suitable peak shape and run time. Confirm that the selected phase is designed for water-rich operation.
Control pH to manage ionization and retention. Use an appropriate buffer where needed and verify compatibility with the detector, especially for LC–MS.
When reversed-phase retention remains insufficient, evaluate a polar-retention mode such as HILIC within the Fusion portfolio, using careful control of water content and sample solvent.
View product information, selection guidance and available configurations.
View product information, selection guidance and available configurations.
View product information, selection guidance and available configurations.
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